In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses
Author(s):
Itoh Y (Itoh, Yasushi)
1,
2,
Shinya K (Shinya, Kyoko),
Kiso M (Kiso, Maki)
3,
Watanabe T (Watanabe, Tokiko)
4,
Sakoda Y (Sakoda, Yoshihiro)
5,
Hatta M (Hatta, Masato)
4,
Muramoto Y (Muramoto, Yukiko)
6,
Tamura D (Tamura, Daisuke)
3,
Sakai-Tagawa Y (Sakai-Tagawa, Yuko)
3,
Noda T (Noda, Takeshi)
7,
Sakabe S (Sakabe, Saori)
3,
Imai M (Imai, Masaki)
4,
Hatta Y (Hatta, Yasuko)
4,
Watanabe S (Watanabe, Shinji)
4,
Li CJ (Li, Chengjun)
4,
Yamada S (Yamada, Shinya)
3,
Fujii K (Fujii, Ken)
6,
Murakami S (Murakami, Shin)
3,
Imai H (Imai, Hirotaka)
3,
Kakugawa S (Kakugawa, Satoshi)
3,
Ito M (Ito, Mutsumi)
3,
Takano R (Takano, Ryo)
3,
Iwatsuki-Horimoto K (Iwatsuki-Horimoto, Kiyoko)
3,
Shimojima M (Shimojima, Masayuki)
3,
Horimoto T (Horimoto, Taisuke)
3,
Goto H (Goto, Hideo)
3,
Takahashi K (Takahashi, Kei)
3,
Makino A (Makino, Akiko)
1,
Ishigaki H (Ishigaki, Hirohito)
2,
Nakayama M (Nakayama, Misako)
2,
Okamatsu M (Okamatsu, Masatoshi)
5,
Takahashi K (Takahashi, Kazuo)
8,
Warshauer D (Warshauer, David)
9,
Shult PA (Shult, Peter A.)
9,
Saito R (Saito, Reiko)
10,
Suzuki H (Suzuki, Hiroshi)
10,
Furuta Y (Furuta, Yousuke)
11,
Yamashita M (Yamashita, Makoto)
12,
Mitamura K (Mitamura, Keiko)
13,
Nakano K (Nakano, Kunio)
13,
Nakamura M (Nakamura, Morio)
13,
Brockman-Schneider R (Brockman-Schneider, Rebecca)
14,
Mitamura H (Mitamura, Hiroshi)
15,
Yamazaki M (Yamazaki, Masahiko)
16,
Sugaya N (Sugaya, Norio)
17,
Suresh M (Suresh, M.)
4,
Ozawa M (Ozawa, Makoto)
4,
Neumann G (Neumann, Gabriele)
4,
Gern J (Gern, James)
14,
Kida H (Kida, Hiroshi)
5,
Ogasawara K (Ogasawara, Kazumasa)
2,
Kawaoka Y (Kawaoka, Yoshihiro)
1,
3,
4,
6,
7,
18
Abstract: Influenza A viruses cause recurrent outbreaks at local or global scale with potentially severe consequences for human health and the global economy. Recently, a new strain of influenza A virus was detected that causes disease in and transmits among humans, probably owing to little or no pre-existing immunity to the new strain. On 11 June 2009 the World Health Organization declared that the infections caused by the new strain had reached pandemic proportion. Characterized as an influenza A virus of the H1N1 subtype, the genomic segments of the new strain were most closely related to swine viruses(1). Most human infections with swine-origin H1N1 influenza viruses (S-OIVs) seem to be mild; however, a substantial number of hospitalized individuals do not have underlying health issues, attesting to the pathogenic potential of S-OIVs. To achieve a better assessment of the risk posed by the new virus, we characterized one of the first US S-OIV isolates, A/California/04/09 ( H1N1; hereafter referred to as CA04), as well as several other S-OIV isolates, in vitro and in vivo. In mice and ferrets, CA04 and other S- OIV isolates tested replicate more efficiently than a currently circulating human H1N1 virus. In addition, CA04 replicates efficiently in non-human primates, causes more severe pathological lesions in the lungs of infected mice, ferrets and non-human primates than a currently circulating human H1N1 virus, and transmits among ferrets. In specific-pathogen-free miniature pigs, CA04 replicates without clinical symptoms. The assessment of human sera from different age groups suggests that infection with human H1N1 viruses antigenically closely related to viruses circulating in 1918 confers neutralizing antibody activity to CA04. Finally, we show that CA04 is sensitive to approved and experimental antiviral drugs, suggesting that these compounds could function as a first line of defence against the recently declared S- OIV pandemic.
Document Type: Article
Language: English
KeyWords Plus: RESISTANT INFLUENZA; A VIRUSES; CELLS; INFECTION; TRANSMISSION; MODEL
Reprint Address: Kawaoka, Y (reprint author), Kobe Univ, Dept Microbiol & Infect Dis, Kobe, Hyogo 6500017 Japan
Source: NATURE Volume: 460 Issue: 7258 Pages: 1021-U110 Published: 2009
Addresses:
1. Kobe Univ, Dept Microbiol & Infect Dis, Kobe, Hyogo 6500017 Japan
2. Shiga Univ Med Sci, Dept Pathol, Shiga 5202192, Japan
3. Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Virol, Tokyo 1088639, Japan
4. Univ Wisconsin, Dept Pathobiol Sci, Madison, WI 53711 USA
5. Hokkaido Univ, Grad Sch Vet Med, Dept Dis Control, Sapporo, Hokkaido 0600818 Japan
6. ERATO Infect Induced Host Responses Project, Kawaguchi, Saitama 3320012 Japan
7. Univ Tokyo, Inst Med Sci, Int Res Ctr Infect Dis, Dept Special Pathogens, Tokyo 1088639, Japan
8. Osaka Prefectural Inst Publ Hlth, Dept Infect Dis, Osaka 5370025, Japan
9. Wisconsin State Lab Hyg, Madison, WI 53706 USA
10. Niigata Univ, Grad Sch Med & Dent Sci, Dept Publ Hlth, Niigata 9518510, Japan
11. Toyama Chem Co Ltd, Toyama 9308508, Japan
12. Daiichi Sankyo Co Ltd, Tokyo 1408710, Japan
13. Eiju Gen Hosp, Tokyo 1108654, Japan
14. Univ Wisconsin, Sch Med & Publ Hlth, Madison, WI 53792 USA
15. Mitamura Clin, Dept Internal Med, Shizuoka 4130103, Japan
16. Childrens Clin, Dept Pediat, Kanagawa 2280023, Japan
17. Keiyu Hosp, Kanagawa 2200012, Japan
18. Hokkaido Univ, Creat Res Initiat, Sapporo, Hokkaido 0600818 Japan E-mail Addresses:
kawaokay@svm.vetmed.wisc.edu